PROCEDURE Between January and March 1996, sexually mature winter flounder were captured from Shark River, NJ by Steve Teluvech of Richard Stockton College Nacote Creek Marine Science Research Center. The flounder were then kept in an aerated tank with a maintained temperature between 1-10(C and a salinity between 11.4 - thirty-three ppt in the said research center. Each individual flounder was injected with a prepared carp pituitary hormone extract (GtH) according to their body weights to …showed first 75 words of 1292 total…

…showed last 75 words of 1292 total…conclusion, the GtH hormone and the experimental protocol were successful in artificial spawning and rearing of winter flounder. To get optimal results, the following conditions are suggested: Aeration must be controlled to maintain a constant oxygen level in the water and to reduce agitation to the developing eggs. Temperature must be maintained within a range of 1-6(C, for optimal egg development. Salinity should be maintained within 20 to 30 ppt. Dry method of fertilization is recommended.